Reponses biochimique et physiologique de la Luzerne arborescente

L'extraction de la proline s'est faite à l'éthanol . selon la méthode de l'AOAC (Association of . Official Analytical Chemists, 1955) modifiée par . Nguyen et Paquin (1971) utilisant .

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Estimation of Proline | Plants

ADVERTISEMENTS: In this article we will discuss about the tests for estimation of proline in plants. Principle: During selective extraction with aqueous sulphosalicylic acid, proteins are precipitated as a complex. Other interfering materials are also presumably removed by absorption to the protein-sulphosalicylic acid complex. The extracted proline is made to react with …

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Full article: Variation saisonnière de la teneur en proline et …

Extraction et dosage de la proline. L'extraction de la proline s'est faite à l'éthanol selon la méthode de l'AOAC (Association of Official Analytical Chemists, Citation 1955) modifiée par Nguyen & Paquin (Citation 1971) utilisant successivement de l'éthanol 95%, de l'éthanol 70% et du chloroforme à froid.

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Comparative analyses of universal extraction buffers for …

Significant differences for protein extraction were detected via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis (2-DE). Between the two universal extraction buffers, the USPB showed higher efficiency for extraction of soluble protein, , GR, O2∙-, GPX, SOD, and free proline, while ...

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Determination of Free Proline in Plants | SpringerLink

Thus, proline biosynthesis would reduce ROS production at the chloroplast level, whereas proline degradation could induce mitochondrial ROS. 1.3 Proline Extraction and Quantification Methods in Plants. Over the last 70 years, multiple methods to extract and quantify proline from different plant tissues have been used and reported in the literature.

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Étude, par une méthode enzymatique, des isomères de la l-proline …

En utilisant la spécificité de la procollagène-proline-hydroxylase pour substituer par un hydroxyle l'hydrogène en position trans sur le carbone 4 de la l-proline procollagénique, nous avons montré que la l-proline [3 H-4] produite par le C.E.N. Saclay contenait 29 p. cent de forme trans et 71 p. cent de forme cis.Il a été également possible de vérifier la stabilité de cette …

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Méthodes d'extraction et de purification des acides aminés …

Méthodes d'extraction et de purification des acides aminés libres et des protéines de tissus végétaux ... an&in&s Acidc aspartique Thrdonine SBrinc Asparagine Acicle' glutamiquc Glutamine Proline Glycinc Alan&' Acicle a-aminobutyriquc Cystinc Valine Mkhionine Mdthionine + mdthionine Isoleucine LFucjnk Tyrosine l?h&nylalaninc Acicle y ...

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Determination of Proline in Human Serum by a Robust LC …

where Response post-extraction spike is the mean peak area count for the proline spiked into extracted matrix after the extraction procedure, and Response matrix-free spike is for the same …

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Extraction and determination of proline – …

Proline and total amino acids may also be extracted using a cold extraction procedure by mixing 20-50 mg fresh weight aliquots with 0.4-1 ml of ethanol:water (40:60 v/v). The resulting mixture is left overnight a 4 ∘ C, and then centrifuged …

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(PDF) Free Proline Quantification

to the de novo proline synthesis in plants subjected to water stress, in a. process dependent of ABA concentration. ... Extraction. 4 m L toluene ar e added to each tube, and vortexed f o r 15-20 s.

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Methods for Determination of Proline in Plants

1.2 Proline Determination with Colorimetric Assay. Proline content of plant tissues can be determined by several methods. In 1952, Chinard published that at acidic pH, ninhydrin can form a red product with proline and ornithine …

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La proline, un acide aminé multifonctionnel impliqué …

m´etabolisme de la proline en absence de contraintes chez A. thaliana (Thiery et al., 2004) tandis que les PLCs, via la signalisation calcique, r´egulent positive-ment la biosynth`ese de la proline lors d'une contrainte saline (Parre et al., 2007). R´ecemment, Ghars et al. (2008a, 2012)ontmisen´evidence l'implication

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Hydroxyproline

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Action de la salinité sur les teneurs en proline des …

L'extraction et le dosage retenus son! decrits dans !'article precedent (Belkhodja et Ait-Sadi, 1993). RESULTATS I - Evolution de Ia proline dans les feuilles, les tiges et les racines au cours du developpement de Ia plante Les figures I, 2 et 3 montrent Ia cineti­ que d'evolution de Ia proline dans les trois organes de Ia variete locale L (I ...

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Proline Protocol: Bates Et Al. (1973) : G Proline/ml X ML

1. The Proline Protocol outlines a procedure for quantifying proline levels in plant tissues. Leaf tissue is ground and homogenized in sulfosalicylic acid. 2. The homogenate is reacted with acid ninhydrin and glacial acetic acid, boiled, cooled, and mixed with toluene to create a chromophore. 3. The absorbance of the chromophore is measured and proline content is determined using a …

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Methods for Determination of Proline in Plants

(c) Proline accumulation in wild-type and p5cs1 mutant Arabidopsis plants treated with saline solutions (0.2 M for 2 or 7 days). (d) Calibration curve for semiquantitative analysis of proline content.

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Estimation of proline | PPT

3. Proline - Introduction Proline also protects the structure of membranes and cellular proteins. Many workers have reported several- fold increase in the proline content under physiological and pathological stress conditions. Hence, the analysis of proline is important for assessing the role of proline in physiological and pathological conditions of plants.

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A detailed explanation for the calculation of proline by Bates …

In the paper there is a formula to convert from your concentrations obtained via the standard curve to µmoles proline/g of fresh weight material: [(µg proline/ml) x ml toluene)/115.5 µg/µmole ...

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Procédé d'extraction et de purification de …

La présente invention concerne un procédé d'extraction acide à faible température et de purification de collagène marin natif type I soluble à partir des écailles de sardine (sardina pilchardus) fraîches ou congelées, caractérisée en …

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Methods for determination of proline in plants

Accumulation of proline in higher plants is an indication of disturbed physiological condition, triggered by biotic or abiotic stress condition. Free proline content can increase upon exposure of plants to drought, salinity, cold, heavy metals, or certain pathogens. Determination of …

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La teneur en proline et la résistance des plantes aux sels

tive (2 ml de m6thanol 40%; 60 rain d'extraction /~ une temp6rature do 85 ~ dans un bain-marie). Selon la teneur en proline 1 ml, 0,5 ml ou 0,25 mlont 6t6 utilisds pour le ... formation de la proline commence ~ une concentration de NaC1 plus dlevte (213 mmol) (figure 3). Ce comportement est particuli~rement net sur la tige qui servira donc d ...

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Observations sur une méthode de dosage de la proline libre …

Folin-type resins used to purify plant extracts of basic amino acids that interfere with proline determination are not selective and partially retain this amino acid and the basic amino acids. Therefore, their use should be avoided, otherwise correction factors must be introduced. Modifications to another method allow a more rapid and precise estimation of proline.

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Les multiples fonctions de la proline dans les plantes …

Download scientific diagram | Les multiples fonctions de la proline dans les plantes (Szabados et Savouré., 2010). La proline est utilisée pour la synthèse des protéines, elle a des fonctions ...

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(PDF) PROLINE ASSAY PROTOCOL

L'addition de L-proline au milieu de culture (quelles que soient les concentrations testées) stimule la croissance des cultures, même en présence de 200 mM de NaCl. La libération de potassium intracellulaire est inversement corrélée à la …

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PROTOCOL: Extraction and determination of …

Proline and total amino acids may also be extracted using a cold extraction procedure by mixing 20-50 mg fresh weight aliquots with 0.4-1 ml of …

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Action de la salinité sur les teneurs en proline des …

tendant l'extraction et le dosage de Ia proline. L'extraction at Ia pur~ication de Ia proline sent reali­ sees a !'ethanol salon Ia methode de I'A.O.A.C. (Asso­ ciation of Official Analytical Chemists) modifi8e par Nguyen at Paquin (1971). Le dosage a fait appal a Ia technique de Singh et a/. (1973). La densite optique de Ia proline est lue a ...

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‪Petronia " Carillo‬

‪University of Campania "Luigi Vanvitelli"‬ - ‪‪8.776 citazioni‬‬ - ‪My research interests are primarily centred on the role of compatible osmolytes in stress responses‬ - ‪plant metabolic profiling‬

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PROTOCOL: Extraction and determination of proline

pipette up to 50 μl ethanolic extract or 50 μl of 1-0.4-0.2-0.1-0.04 mM proline standard prepared in 70:30 ethanol:water (v/v) seal the tubes, mix and heat at 95°C (either in block heater or ...

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Use of magnetic ionic liquid for selective extraction of proline …

In the determination of proline in honey samples, different analytical methods are used such as fluorimetry (Paramás et al., 2006), liquid chromatography - mass spectrometry (LC-MS) (Rebane & Herodes, 2010), high-resolution liquid chromatography (Ouchemoukh et al., 2010) gas chromatography (GC) (De La Fuente et al., 2011), capillary ...

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Centrifugeuse Proline JE400

Avec son design compact et élégant, la centrifugeuse JE400 de proline trouvera facilement sa place sur n'importe quel plan de travail. Performances : Doté d'une puissance de 400 watts, la centrifugeuse de Proline vous permet de préparer des jus délicieux sans effort.

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